LymphedemaV1, Main, Exploration, bibRecord, 00A190

The Abundant Larval Transcript-1 and -2 Genes of Brugia malayi Encode Stage-Specific Candidate Vaccine Antigens for Filariasis

Identifieur interne : 00A190 ( Main/Exploration ); précédent : 00A189; suivant : 00A191

The Abundant Larval Transcript-1 and -2 Genes of Brugia malayi Encode Stage-Specific Candidate Vaccine Antigens for Filariasis

Auteurs : William F. Gregory ; Agnes K. Atmadja ; Judith E. Allen ; Rick M. Maizels

Source :

Infection and Immunity [ 0019-9567 ] ; 2000.

RBID : PMC:101719

Descripteurs français

KwdFr :
- Amorces ADN (génétique), Animaux, Antigènes d'helminthe (génétique), Brugia malayi (croissance et développement), Brugia malayi (génétique), Brugia malayi (immunologie), Caenorhabditis elegans (génétique), Caenorhabditis elegans (immunologie), Données de séquences moléculaires, Filariose lymphatique (), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Gerbillinae, Humains, Immunisation, Introns, Larve (génétique), Larve (immunologie), Mâle, Protéines d'helminthes (génétique), Protéines d'helminthes (immunologie), Régulation de l'expression des gènes au cours du développement, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Vaccins (génétique), Variation génétique.
MESH :
- croissance et développement : Brugia malayi.
- génétique : Amorces ADN, Antigènes d'helminthe, Brugia malayi, Caenorhabditis elegans, Larve, Protéines d'helminthes, Vaccins.
- immunologie : Brugia malayi, Caenorhabditis elegans, Filariose lymphatique, Larve, Protéines d'helminthes.
- parasitologie : Filariose lymphatique.
- Animaux, Données de séquences moléculaires, Filariose lymphatique, Gerbillinae, Humains, Immunisation, Introns, Mâle, Régulation de l'expression des gènes au cours du développement, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Variation génétique.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antigens, Helminth (genetics), Base Sequence, Brugia malayi (genetics), Brugia malayi (growth & development), Brugia malayi (immunology), Caenorhabditis elegans (genetics), Caenorhabditis elegans (immunology), DNA Primers (genetics), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Elephantiasis, Filarial (prevention & control), Gene Expression Regulation, Developmental, Genetic Variation, Gerbillinae, Helminth Proteins (genetics), Helminth Proteins (immunology), Humans, Immunization, Introns, Larva (genetics), Larva (immunology), Male, Molecular Sequence Data, Sequence Homology, Amino Acid, Vaccines (genetics).
MESH :
- chemical , genetics : Antigens, Helminth, DNA Primers, Helminth Proteins, Vaccines.
- genetics : Brugia malayi, Caenorhabditis elegans, Larva.
- growth & development : Brugia malayi.
- immunology : Brugia malayi, Caenorhabditis elegans, Elephantiasis, Filarial, Helminth Proteins, Larva.
- parasitology : Elephantiasis, Filarial.
- prevention & control : Elephantiasis, Filarial.
- Amino Acid Sequence, Animals, Base Sequence, Gene Expression Regulation, Developmental, Genetic Variation, Gerbillinae, Humans, Immunization, Introns, Male, Molecular Sequence Data, Sequence Homology, Amino Acid.

Abstract

Lymphatic filariasis is a major tropical disease caused by the mosquito-borne nematodes Brugia and Wuchereria. About 120 million people are infected and at risk of lymphatic pathology such as acute lymphangitis and elephantiasis. Vaccines against filariasis must generate immunity to the infective mosquito-derived third-stage larva (L3) without accentuating immunopathogenic responses to lymphatic-dwelling adult parasites. We have identified two highly expressed genes, designated abundant larval transcript-1 and -2 (alt-1 and alt-2), from each of which mRNAs account for >1% of L3 cDNAs. ALT-1 and ALT-2 share 79% amino acid identity across 125 residues, including a putative signal sequence and a prominent acidic tract. Expression of alt-1 and alt-2 is initiated midway through development in the mosquito, peaking in the infective larva and declining sharply following entry into the host. Humans exposed to Brugia malayi show a high frequency of immunoglobulin G1 (IgG1) and IgG3 antibodies to ALT-1 and -2, distinguishing them from adult-stage antigens, which are targeted by the IgG4 isotype. Immunization of susceptible rodents (jirds) with ALT-1 elicited a 76% reduction in parasite survival, the highest reported for a single antigen from any filarial parasite. ALT-1 and the closely related ALT-2 are therefore strong candidates for a future vaccine against human filariasis.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC101719

PubMed: 10858234
PubMed Central: 101719

Affiliations:

Le document en format XML

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 Encode Stage-Specific Candidate  Vaccine Antigens for Filariasis</title>
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 Encode Stage-Specific Candidate  Vaccine Antigens for Filariasis</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antigens, Helminth (genetics)</term>
<term>Base Sequence</term>
<term>Brugia malayi (genetics)</term>
<term>Brugia malayi (growth & development)</term>
<term>Brugia malayi (immunology)</term>
<term>Caenorhabditis elegans (genetics)</term>
<term>Caenorhabditis elegans (immunology)</term>
<term>DNA Primers (genetics)</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Elephantiasis, Filarial (parasitology)</term>
<term>Elephantiasis, Filarial (prevention & control)</term>
<term>Gene Expression Regulation, Developmental</term>
<term>Genetic Variation</term>
<term>Gerbillinae</term>
<term>Helminth Proteins (genetics)</term>
<term>Helminth Proteins (immunology)</term>
<term>Humans</term>
<term>Immunization</term>
<term>Introns</term>
<term>Larva (genetics)</term>
<term>Larva (immunology)</term>
<term>Male</term>
<term>Molecular Sequence Data</term>
<term>Sequence Homology, Amino Acid</term>
<term>Vaccines (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (génétique)</term>
<term>Animaux</term>
<term>Antigènes d'helminthe (génétique)</term>
<term>Brugia malayi (croissance et développement)</term>
<term>Brugia malayi (génétique)</term>
<term>Brugia malayi (immunologie)</term>
<term>Caenorhabditis elegans (génétique)</term>
<term>Caenorhabditis elegans (immunologie)</term>
<term>Données de séquences moléculaires</term>
<term>Filariose lymphatique ()</term>
<term>Filariose lymphatique (immunologie)</term>
<term>Filariose lymphatique (parasitologie)</term>
<term>Gerbillinae</term>
<term>Humains</term>
<term>Immunisation</term>
<term>Introns</term>
<term>Larve (génétique)</term>
<term>Larve (immunologie)</term>
<term>Mâle</term>
<term>Protéines d'helminthes (génétique)</term>
<term>Protéines d'helminthes (immunologie)</term>
<term>Régulation de l'expression des gènes au cours du développement</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Vaccins (génétique)</term>
<term>Variation génétique</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term>
<term>DNA Primers</term>
<term>Helminth Proteins</term>
<term>Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Brugia malayi</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term>
<term>Caenorhabditis elegans</term>
<term>Larva</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Brugia malayi</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term>
<term>Antigènes d'helminthe</term>
<term>Brugia malayi</term>
<term>Caenorhabditis elegans</term>
<term>Larve</term>
<term>Protéines d'helminthes</term>
<term>Vaccins</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Brugia malayi</term>
<term>Caenorhabditis elegans</term>
<term>Filariose lymphatique</term>
<term>Larve</term>
<term>Protéines d'helminthes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term>
<term>Caenorhabditis elegans</term>
<term>Elephantiasis, Filarial</term>
<term>Helminth Proteins</term>
<term>Larva</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Gene Expression Regulation, Developmental</term>
<term>Genetic Variation</term>
<term>Gerbillinae</term>
<term>Humans</term>
<term>Immunization</term>
<term>Introns</term>
<term>Male</term>
<term>Molecular Sequence Data</term>
<term>Sequence Homology, Amino Acid</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Filariose lymphatique</term>
<term>Gerbillinae</term>
<term>Humains</term>
<term>Immunisation</term>
<term>Introns</term>
<term>Mâle</term>
<term>Régulation de l'expression des gènes au cours du développement</term>
<term>Similitude de séquences d'acides aminés</term>
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<front><div type="abstract" xml:lang="en"><p>Lymphatic filariasis is a major tropical disease caused by the mosquito-borne nematodes <italic>Brugia</italic>
 and <italic>Wuchereria</italic>
. About 120 million people are infected and at risk of lymphatic pathology such as acute lymphangitis and elephantiasis. Vaccines against filariasis must generate immunity to the infective mosquito-derived third-stage larva (L3) without accentuating immunopathogenic responses to lymphatic-dwelling adult parasites. We have identified two highly expressed genes, designated abundant larval transcript-1 and -2 (<italic>alt-1</italic>
 and <italic>alt-2</italic>
), from each of which mRNAs account for >1% of L3 cDNAs. ALT-1 and ALT-2 share 79% amino acid identity across 125 residues, including a putative signal sequence and a prominent acidic tract. Expression of <italic>alt-1</italic>
 and <italic>alt-2</italic>
 is initiated midway through development in the mosquito, peaking in the infective larva and declining sharply following entry into the host. Humans exposed to <italic>Brugia malayi</italic>
 show a high frequency of immunoglobulin G1 (IgG1) and IgG3 antibodies to ALT-1 and -2, distinguishing them from adult-stage antigens, which are targeted by the IgG4 isotype. Immunization of susceptible rodents (jirds) with ALT-1 elicited a 76% reduction in parasite survival, the highest reported for a single antigen from any filarial parasite. ALT-1 and the closely related ALT-2 are therefore strong candidates for a future vaccine against human filariasis.</p>
</div>
</front>
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<tree><noCountry><name sortKey="Allen, Judith E" sort="Allen, Judith E" uniqKey="Allen J" first="Judith E." last="Allen">Judith E. Allen</name>
<name sortKey="Atmadja, Agnes K" sort="Atmadja, Agnes K" uniqKey="Atmadja A" first="Agnes K." last="Atmadja">Agnes K. Atmadja</name>
<name sortKey="Gregory, William F" sort="Gregory, William F" uniqKey="Gregory W" first="William F." last="Gregory">William F. Gregory</name>
<name sortKey="Maizels, Rick M" sort="Maizels, Rick M" uniqKey="Maizels R" first="Rick M." last="Maizels">Rick M. Maizels</name>
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Serveur d'exploration sur le lymphœdème

The Abundant Larval Transcript-1 and -2 Genes of Brugia malayi Encode Stage-Specific Candidate Vaccine Antigens for Filariasis

The Abundant Larval Transcript-1 and -2 Genes of Brugia malayi Encode Stage-Specific Candidate Vaccine Antigens for Filariasis

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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